This volume and its companion Volume 351 supplements Volume 194 of MIE. The guides are specifically designed to meet the needs of graduate students and postdocs as well as researchers. Whether an established researcher or newcomer to the field, these volumes contain the up-to-date methods needed to study 'Genes in Yeast.' Procedures are included to enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information is given for proven procedures that can be used as a guide for developing protocols in a number of disciplines.