This fourth edition of the successful laboratory guide which has provided a rich story of riboneucleic acid for over fifteen years represents a growing collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA. With greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites, this edition provides investigators with the essential information to optimize time at the bench. The abundant use of flow charts, tables, graphs and insightful text provide useful analysis with clear take-home lessons especially helpful in the planning and implementation phases of a project.Author Info