PCR

PCR is probably the most widely-used technique in molecular biology today, with new variants appearing on a frequent basis. Many of the new PCR methods offer substantial benefits over protocols that have previously been covered in original papers and lab manuals. 'PCR: Methods Express' describes the latest PCR-based methodologies and approaches and provides the most up-to-date practical advice on how to tackle a broad range of biological problems using these techniques, including: quantitative RT-PCR; identification of genomic micro-deletions and duplications; DNA methylation analysis; DNA typing for forensic genetics; rapid generation of gene targeting constructs; pre-implantation genetic disease diagnosis; construction of long DNA molecules from multiple fragments; and identification of low level mutations in tumors. 'PCR: Methods Express' is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategies for optimization. This book is an essential laboratory manual for researchers in all biomedical fields, and at all levels, from postgraduate student to principal investigator.